RESUMO
In order to ascertain the presence and the in muro localization of cellulose, the enzyme-gold affinity test was applied to algal cell walls. The high specificity of affinity cytochemistry allowed us, by using the enzyme cellulase, to confirm the available biochemical data and to give a map of the cellulose localization in different algal groups. Taking into account the complex skeletal polysaccharide structure and composition of the algal cell walls, this method proved to be a reliable tool in this field.
Assuntos
Parede Celular/química , Celulose/análise , Eucariotos/química , Parede Celular/ultraestrutura , Celulose/ultraestrutura , Eucariotos/ultraestrutura , Ouro , Histocitoquímica , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Coloração e RotulagemRESUMO
We have identified diatoms among other thermophilic microorganisms as the main agents for the colonization of thermal mud resulting in a 'maturation' which renders the mud suitable to be used for mud-pack treatment of osteoarthrosis patients. The main effects of the diatom growth are the progressive enrichment of mud extracts in chlorophyll a parallel to the building up of a sulfoglycolipid endowed with an anti-inflammatory action. The sulfoglycolipid was also produced by diatoms isolated from the mud and cultivated in vitro.
